Journal: BMC Veterinary Research

Article Title: Immunity against a Japanese local strain of porcine reproductive and respiratory syndrome virus decreases viremia and symptoms of a highly pathogenic strain

doi: 10.1186/s12917-021-02863-4

Figure Lengend Snippet: Alignment of the amino acid sequences of the nsp2 gene from divergent porcine reproductive and respiratory syndrome virus (PRRSV) strains. Genetic analysis was conducted using CLUSTAL W with Genetyx Software v14 (Genetyx, Tokyo, Japan). The black boxes indicate complete amino acid conservation (4/4), whereas gray and unshaded boxes identify less conserved residues (3/4 or less). A signal peptide was predicted using the Genetyx Software v14 (Genetyx, Tokyo, Japan) (yellow-boxed region). The completely conserved putative cysteine–protease catalytic residues are identified by the red-boxed amino acids to signify protease sequence conservation within PRRSV and equine arteritis virus. The potential N -glycosylation sites indicated by asterisks (*) were identified by PROSITE . The conserved cysteine residue that is proposed to interact with the M protein is denoted by the downward arrow (↓)

Article Snippet: Genetic analysis was conducted using CLUSTAL W with Genetyx Software v14 (Genetyx, Tokyo, Japan).

Techniques: Software, Sequencing

Journal:

Article Title: Molecular Characterization of Porcine Rotaviruses from the Southern Region of Brazil: Characterization of an Atypical Genotype G[9] Strain

doi:

Figure Lengend Snippet: Phylogenetic analysis of the VP7 gene sequences of porcine (ICB2185, Gottfried, OSU, and YM) and human (M3014, ST3, 116E, F45, and WI61) rotavirus strains. The dendrogram was constructed by the Clustal method using the Lasergene sequence analysis software. The length of each pair of branches represents the distance between sequence pairs, while the units at the bottom of the tree indicate the number of substitution events.

Article Snippet: The dendrogram was constructed by the Clustal method using the Lasergene sequence analysis software.

Techniques: Construct, Sequencing, Software